Trehalose synthase of Mycobacterium smegmatis
نویسندگان
چکیده
Yuan T. Pan, Vineetha Koroth Edavana, William J. Jourdian, Rick Edmondson, J. David Carroll, Irena Pastuszak and Alan D. Elbein Department of Biochemistry and Molecular Biology and Department of Microbiology and Immunology, University of Arkansas for Medical Sciences, Little Rock, AR, USA; Departments of Biological Chemistry and Internal Medicine, University of Michigan Medical Center, Ann Arbor, MI, USA; National Center for Toxicological Research, Jefferson, AR, USA
منابع مشابه
Flux through Trehalose Synthase Flows from Trehalose to the Alpha Anomer of Maltose in Mycobacteria
Trehalose synthase (TreS) was thought to catalyze flux from maltose to trehalose, a precursor of essential trehalose mycolates in mycobacterial cell walls. However, we now show, using a genetic approach, that TreS is not required for trehalose biosynthesis in Mycobacterium smegmatis, whereas two alternative trehalose-biosynthetic pathways (OtsAB and TreYZ) are crucial. Consistent with this dire...
متن کاملMechanistic analysis of trehalose synthase from Mycobacterium smegmatis.
Trehalose synthase (TreS) catalyzes the reversible interconversion of maltose and trehalose and has been shown recently to function primarily in the mobilization of trehalose as a glycogen precursor. Consequently, the mechanism of this intriguing isomerase is of both academic and potential pharmacological interest. TreS catalyzes the hydrolytic cleavage of α-aryl glucosides as well as α-glucosy...
متن کاملTrehalose is required for growth of Mycobacterium smegmatis.
Mycobacteria contain high levels of the disaccharide trehalose in free form as well as within various immunologically relevant glycolipids such as cord factor and sulfolipid-1. By contrast, most bacteria use trehalose solely as a general osmoprotectant or thermoprotectant. Mycobacterium tuberculosis and Mycobacterium smegmatis possess three pathways for the synthesis of trehalose. Most bacteria...
متن کاملTrehalose Is Required for Growth of Mycobacterium smegmatis*□S
Mycobacteria contain high levels of the disaccharide trehalose in free form as well as within various immunologically relevant glycolipids such as cord factor and sulfolipid-1. By contrast, most bacteria use trehalose solely as a general osmoprotectant or thermoprotectant. Mycobacterium tuberculosis and Mycobacterium smegmatis possess three pathways for the synthesis of trehalose. Most bacteria...
متن کاملPurification and characterization of a novel mycolic acid exchange enzyme from Mycobacterium smegmatis.
We have isolated and purified to homogeneity an alpha,alpha'-trehalose 6-monomycolate:alpha,alpha'-trehalose mycolyltransferase (trehalose mycolyltransferase) from Mycobacterium smegmatis that catalyzes the exchange of a mycolyl group between trehalose, trehalose 6-monomycolate (TM), and trehalose 6,6'-dimycolate (TD). This enzyme was prominent in M. smegmatis and it catalyzed the following rea...
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